One of the most fertile applications of next generation sequencing technology will be in the field of cancer genomics. Here, we report a high-throughput multi-dimensional sequencing study of primary non-small cell lung adenocarcinoma tumors and adjacent normal tissues of 6 never-smoker Korean female patients. Our data encompass results from exome-seq, RNA-seq, small RNA-seq, and MeDIP-seq. We identified and validated novel genetic aberrations including 47 somatic mutations and 20 fusion transcripts. We also characterized gene expression profiles which we sought to integrate with genomic aberrations and epigenetic regulations into functional networks. Importantly, several gene network modules relevant to carcinogenesis including G2/M cell cycle checkpoint emerged as the primary sources of disturbance in these patients. In addition, the data strongly suggest that microRNAs make key regulatory inputs into these modules. Our study offers a paradigm for integrative genomics analysis and proposes potential target pathways for the control of non-small cell lung adenocarcinoma.
Supp. Fig. 1. Overview of experimental data and analysis